pebmulti-neo vector Search Results


pebmulti-vector  (FUJIFILM)
90
FUJIFILM pebmulti-vector
Pebmulti Vector, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pebmulti-neo+vector/pebmulti+neo+vector/pmc04830023-66-15-16
Average 90 stars, based on 1 article reviews
pebmulti-vector - by Bioz Stars, 2026-09
90/100 stars
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pebmulti neo lrg1 vector  (FUJIFILM)
90
FUJIFILM pebmulti neo lrg1 vector
Effect of LRG on TGF ‐ β ‐induced Smad2 phosphorylation and downstream gene expression in L929. (A) Silver staining of affinity‐purified recombinant mouse LRG obtained from the culture supernatant of recombinant mouse LRG ‐expressing A549 cells using an anti‐ LRG antibody‐conjugated column. The sizes of molecular weight markers are indicated on the right lane. The size of the major band (indicated by arrowhead) corresponded to that of LRG (45–50 kD a). (B and C) Detection of phospho‐Smad2 by western blot analysis. L929 cells were treated with or without 20 μ g/ mL of LRG for 24 h. Cells were then stimulated with 2 ng/ mL of TGF ‐ β 1 for 0, 30, and 60 min. Anti‐phospho‐Smad2 (Ser465/467), anti‐Smad2, anti‐ LRG , and anti‐ GAPDH antibodies were used for the detection. Band intensity of pS mad2 normalized to Smad2 is shown in Figure C ( n = 3). (D and E) Detection of phospho‐Smad2 by western blot analysis. L929 cells were treated without (lanes 1 and 2) or with 0.035 μ g/ mL (lane 3), 0.35 μ g/ mL (lane 4), and 3.5 μ g/ mL (lane 5) of LRG for 24 h and then with 2 ng/ mL of TGF ‐ β 1 for 30 min. Band intensity of pS mad2 normalized to Smad2 is shown in Figure E ( n = 3). (F) Detection of LRG in culture supernatants of pc DNA <t>‐Lrg1</t> (containing full length LRG cDNA ) but not in those of pc DNA (vector without insertion). Supernatants from L929 cells after 24‐h culture in serum‐starved media were analyzed by western blot. (G and H) Detection of phospho‐Smad2 in pc DNA ‐Lrg1 and pc DNA cells after treatment with 2 ng/ mL of TGF ‐ β 1 for 0, 30, and 60 min. Band intensity of pS mad2 normalized to Smad2 is shown in Figure H ( n = 3). (I) qPCR analysis of Serpine1 and Acta2 . L929 cells were treated with 2 ng/ mL of TGF ‐ β 1 for 6 h for Serpine1 or for 12 h for Acta2 . The relative expressions of Serpine1 and Acta2 , normalized to Hprt1 gene expression are shown.
Pebmulti Neo Lrg1 Vector, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pebmulti-neo+vector/pebmulti+neo+lrg1+vector/pmc05742708-68-5-7
Average 90 stars, based on 1 article reviews
pebmulti neo lrg1 vector - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
pdk2 stable overexpression peb multi-neo vector  (FUJIFILM)
90
FUJIFILM pdk2 stable overexpression peb multi-neo vector
Effect of LRG on TGF ‐ β ‐induced Smad2 phosphorylation and downstream gene expression in L929. (A) Silver staining of affinity‐purified recombinant mouse LRG obtained from the culture supernatant of recombinant mouse LRG ‐expressing A549 cells using an anti‐ LRG antibody‐conjugated column. The sizes of molecular weight markers are indicated on the right lane. The size of the major band (indicated by arrowhead) corresponded to that of LRG (45–50 kD a). (B and C) Detection of phospho‐Smad2 by western blot analysis. L929 cells were treated with or without 20 μ g/ mL of LRG for 24 h. Cells were then stimulated with 2 ng/ mL of TGF ‐ β 1 for 0, 30, and 60 min. Anti‐phospho‐Smad2 (Ser465/467), anti‐Smad2, anti‐ LRG , and anti‐ GAPDH antibodies were used for the detection. Band intensity of pS mad2 normalized to Smad2 is shown in Figure C ( n = 3). (D and E) Detection of phospho‐Smad2 by western blot analysis. L929 cells were treated without (lanes 1 and 2) or with 0.035 μ g/ mL (lane 3), 0.35 μ g/ mL (lane 4), and 3.5 μ g/ mL (lane 5) of LRG for 24 h and then with 2 ng/ mL of TGF ‐ β 1 for 30 min. Band intensity of pS mad2 normalized to Smad2 is shown in Figure E ( n = 3). (F) Detection of LRG in culture supernatants of pc DNA <t>‐Lrg1</t> (containing full length LRG cDNA ) but not in those of pc DNA (vector without insertion). Supernatants from L929 cells after 24‐h culture in serum‐starved media were analyzed by western blot. (G and H) Detection of phospho‐Smad2 in pc DNA ‐Lrg1 and pc DNA cells after treatment with 2 ng/ mL of TGF ‐ β 1 for 0, 30, and 60 min. Band intensity of pS mad2 normalized to Smad2 is shown in Figure H ( n = 3). (I) qPCR analysis of Serpine1 and Acta2 . L929 cells were treated with 2 ng/ mL of TGF ‐ β 1 for 6 h for Serpine1 or for 12 h for Acta2 . The relative expressions of Serpine1 and Acta2 , normalized to Hprt1 gene expression are shown.
Pdk2 Stable Overexpression Peb Multi Neo Vector, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pebmulti-neo+vector/pdk2+stable+overexpression+peb+multi+neo+vector/pmc04199368-332-0-6
Average 90 stars, based on 1 article reviews
pdk2 stable overexpression peb multi-neo vector - by Bioz Stars, 2026-09
90/100 stars
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peb-multi-neo mammalian expression vector  (FUJIFILM)
90
FUJIFILM peb-multi-neo mammalian expression vector
Effect of LRG on TGF ‐ β ‐induced Smad2 phosphorylation and downstream gene expression in L929. (A) Silver staining of affinity‐purified recombinant mouse LRG obtained from the culture supernatant of recombinant mouse LRG ‐expressing A549 cells using an anti‐ LRG antibody‐conjugated column. The sizes of molecular weight markers are indicated on the right lane. The size of the major band (indicated by arrowhead) corresponded to that of LRG (45–50 kD a). (B and C) Detection of phospho‐Smad2 by western blot analysis. L929 cells were treated with or without 20 μ g/ mL of LRG for 24 h. Cells were then stimulated with 2 ng/ mL of TGF ‐ β 1 for 0, 30, and 60 min. Anti‐phospho‐Smad2 (Ser465/467), anti‐Smad2, anti‐ LRG , and anti‐ GAPDH antibodies were used for the detection. Band intensity of pS mad2 normalized to Smad2 is shown in Figure C ( n = 3). (D and E) Detection of phospho‐Smad2 by western blot analysis. L929 cells were treated without (lanes 1 and 2) or with 0.035 μ g/ mL (lane 3), 0.35 μ g/ mL (lane 4), and 3.5 μ g/ mL (lane 5) of LRG for 24 h and then with 2 ng/ mL of TGF ‐ β 1 for 30 min. Band intensity of pS mad2 normalized to Smad2 is shown in Figure E ( n = 3). (F) Detection of LRG in culture supernatants of pc DNA <t>‐Lrg1</t> (containing full length LRG cDNA ) but not in those of pc DNA (vector without insertion). Supernatants from L929 cells after 24‐h culture in serum‐starved media were analyzed by western blot. (G and H) Detection of phospho‐Smad2 in pc DNA ‐Lrg1 and pc DNA cells after treatment with 2 ng/ mL of TGF ‐ β 1 for 0, 30, and 60 min. Band intensity of pS mad2 normalized to Smad2 is shown in Figure H ( n = 3). (I) qPCR analysis of Serpine1 and Acta2 . L929 cells were treated with 2 ng/ mL of TGF ‐ β 1 for 6 h for Serpine1 or for 12 h for Acta2 . The relative expressions of Serpine1 and Acta2 , normalized to Hprt1 gene expression are shown.
Peb Multi Neo Mammalian Expression Vector, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pebmulti-neo+vector/peb+multi+neo+mammalian+expression+vector/10__1158_slash_0008___5472__can___13___3037-44-21-24
Average 90 stars, based on 1 article reviews
peb-multi-neo mammalian expression vector - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


Effect of LRG on TGF ‐ β ‐induced Smad2 phosphorylation and downstream gene expression in L929. (A) Silver staining of affinity‐purified recombinant mouse LRG obtained from the culture supernatant of recombinant mouse LRG ‐expressing A549 cells using an anti‐ LRG antibody‐conjugated column. The sizes of molecular weight markers are indicated on the right lane. The size of the major band (indicated by arrowhead) corresponded to that of LRG (45–50 kD a). (B and C) Detection of phospho‐Smad2 by western blot analysis. L929 cells were treated with or without 20 μ g/ mL of LRG for 24 h. Cells were then stimulated with 2 ng/ mL of TGF ‐ β 1 for 0, 30, and 60 min. Anti‐phospho‐Smad2 (Ser465/467), anti‐Smad2, anti‐ LRG , and anti‐ GAPDH antibodies were used for the detection. Band intensity of pS mad2 normalized to Smad2 is shown in Figure C ( n = 3). (D and E) Detection of phospho‐Smad2 by western blot analysis. L929 cells were treated without (lanes 1 and 2) or with 0.035 μ g/ mL (lane 3), 0.35 μ g/ mL (lane 4), and 3.5 μ g/ mL (lane 5) of LRG for 24 h and then with 2 ng/ mL of TGF ‐ β 1 for 30 min. Band intensity of pS mad2 normalized to Smad2 is shown in Figure E ( n = 3). (F) Detection of LRG in culture supernatants of pc DNA ‐Lrg1 (containing full length LRG cDNA ) but not in those of pc DNA (vector without insertion). Supernatants from L929 cells after 24‐h culture in serum‐starved media were analyzed by western blot. (G and H) Detection of phospho‐Smad2 in pc DNA ‐Lrg1 and pc DNA cells after treatment with 2 ng/ mL of TGF ‐ β 1 for 0, 30, and 60 min. Band intensity of pS mad2 normalized to Smad2 is shown in Figure H ( n = 3). (I) qPCR analysis of Serpine1 and Acta2 . L929 cells were treated with 2 ng/ mL of TGF ‐ β 1 for 6 h for Serpine1 or for 12 h for Acta2 . The relative expressions of Serpine1 and Acta2 , normalized to Hprt1 gene expression are shown.

Journal: Physiological Reports

Article Title: Leucine‐rich α ‐2 glycoprotein promotes lung fibrosis by modulating TGF‐ β signaling in fibroblasts

doi: 10.14814/phy2.13556

Figure Lengend Snippet: Effect of LRG on TGF ‐ β ‐induced Smad2 phosphorylation and downstream gene expression in L929. (A) Silver staining of affinity‐purified recombinant mouse LRG obtained from the culture supernatant of recombinant mouse LRG ‐expressing A549 cells using an anti‐ LRG antibody‐conjugated column. The sizes of molecular weight markers are indicated on the right lane. The size of the major band (indicated by arrowhead) corresponded to that of LRG (45–50 kD a). (B and C) Detection of phospho‐Smad2 by western blot analysis. L929 cells were treated with or without 20 μ g/ mL of LRG for 24 h. Cells were then stimulated with 2 ng/ mL of TGF ‐ β 1 for 0, 30, and 60 min. Anti‐phospho‐Smad2 (Ser465/467), anti‐Smad2, anti‐ LRG , and anti‐ GAPDH antibodies were used for the detection. Band intensity of pS mad2 normalized to Smad2 is shown in Figure C ( n = 3). (D and E) Detection of phospho‐Smad2 by western blot analysis. L929 cells were treated without (lanes 1 and 2) or with 0.035 μ g/ mL (lane 3), 0.35 μ g/ mL (lane 4), and 3.5 μ g/ mL (lane 5) of LRG for 24 h and then with 2 ng/ mL of TGF ‐ β 1 for 30 min. Band intensity of pS mad2 normalized to Smad2 is shown in Figure E ( n = 3). (F) Detection of LRG in culture supernatants of pc DNA ‐Lrg1 (containing full length LRG cDNA ) but not in those of pc DNA (vector without insertion). Supernatants from L929 cells after 24‐h culture in serum‐starved media were analyzed by western blot. (G and H) Detection of phospho‐Smad2 in pc DNA ‐Lrg1 and pc DNA cells after treatment with 2 ng/ mL of TGF ‐ β 1 for 0, 30, and 60 min. Band intensity of pS mad2 normalized to Smad2 is shown in Figure H ( n = 3). (I) qPCR analysis of Serpine1 and Acta2 . L929 cells were treated with 2 ng/ mL of TGF ‐ β 1 for 6 h for Serpine1 or for 12 h for Acta2 . The relative expressions of Serpine1 and Acta2 , normalized to Hprt1 gene expression are shown.

Article Snippet: A549 cells were transfected with pEBMulti‐Neo‐Lrg1 vector (Wako, Osaka, Japan) to obtain mouse LRG‐expressing cells.

Techniques: Expressing, Silver Staining, Affinity Purification, Recombinant, Molecular Weight, Western Blot, Plasmid Preparation